8K9O
Crystal structure of Cyanobacteriochrome RcaE GAF domain in Pg state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-02-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.1211 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.273, 64.273, 84.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.489 - 1.750 |
| Rwork | 0.188 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ckv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.706 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.489 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.069 | 0.903 |
| Number of reflections | 18446 | 1002 |
| <I/σ(I)> | 26.2 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 14.1 |
| CC(1/2) | 1.000 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, magnesium acetate, sodium cacodylate |
