8K9D

Structure of human Caprin-2 HR1 domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	C 2 2 21
Unit cell lengths	56.852, 281.903, 63.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.240 - 3.300
R-factor	0.2907
Rwork	0.289
R-free	0.30220
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.110	3.110
Rmerge	0.116	0.782
Number of reflections	14540	408
<I/σ(I)>	17.3
Completeness [%]	96.5
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20-25% PEG3350, 1.6M potassium thiocyanate