8K9D
Structure of human Caprin-2 HR1 domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.852, 281.903, 63.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.240 - 3.300 |
| R-factor | 0.2907 |
| Rwork | 0.289 |
| R-free | 0.30220 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.116 | 0.782 |
| Number of reflections | 14540 | 408 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 96.5 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20-25% PEG3350, 1.6M potassium thiocyanate |
