8K9C

Structure of human Caprin-2 HR1 domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	C 1 2 1
Unit cell lengths	139.721, 65.322, 112.203
Unit cell angles	90.00, 113.75, 90.00

Refinement procedure

Resolution	30.210 - 2.320
R-factor	0.2188
Rwork	0.218
R-free	0.23990
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.320	2.350
Rmerge	0.124	0.875
Number of reflections	75003	1885
<I/σ(I)>	31.67	2.34
Completeness [%]	97.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	12-15% PEG3350, 20 mM lithiuym chloride