8K8D

Crystal structure of C/EBPbeta BZIP domain bound to a high affinity DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	63.668, 46.400, 98.783
Unit cell angles	90.00, 108.76, 90.00

Refinement procedure

Resolution	30.130 - 2.200
R-factor	0.2388
Rwork	0.236
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.140	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	13958	608
<I/σ(I)>	15.6
Completeness [%]	99.9
Redundancy	1.9
CC(1/2)	0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.02 M Calcium chloride dihydrate,0.1 M Sodium acetate trihydrate pH 4.6 and 20% v/v 2-Propanol