8K8C

Crystal structure of C/EBPalpha BZIP domain bound to a high affinity DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	39.382, 63.366, 128.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.060
R-factor	0.2308
Rwork	0.226
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.073
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.270	2.170
High resolution limit [Å]	2.060	2.060
Number of reflections	20836	2979
<I/σ(I)>	18.9
Completeness [%]	99.9
Redundancy	12.5
CC(1/2)	1.000	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.015 M Calcium chloride dihydrate,0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v 2-Methyl-2,4-pentanediol