8K6V

LnaB-Actin-PRUb ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979191
Spacegroup name	P 21 21 2
Unit cell lengths	215.964, 55.044, 79.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.750 - 2.600
R-factor	0.2555
Rwork	0.253
R-free	0.30690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.598
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.750	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	30202	2918
<I/σ(I)>	4.7
Completeness [%]	100.0
Redundancy	11
CC(1/2)	0.915	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	Magnesium acetate, MOPS, PEG8000