8K6R

LnaB-Actin-PRUb ternary complex in the presence of AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979191
Spacegroup name	P 21 21 2
Unit cell lengths	217.970, 55.903, 80.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.480 - 2.760
R-factor	0.2091
Rwork	0.205
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.545
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.480	2.860
High resolution limit [Å]	2.760	2.760
Rmerge	0.036
Number of reflections	26085	25650
<I/σ(I)>	13.73
Completeness [%]	98.0
Redundancy	2
CC(1/2)	0.998	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	Sodium Chloride, Sodium HEPES, PEG8000