8K6Q

Crystal structure of HOIL-1L LTM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97890
Spacegroup name	P 1 21 1
Unit cell lengths	44.584, 36.706, 54.581
Unit cell angles	90.00, 95.25, 90.00

Refinement procedure

Resolution	44.400 - 1.590
R-factor	0.1813
Rwork	0.180
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	1.650
High resolution limit [Å]	1.590	1.590
Number of reflections	21915	1379
<I/σ(I)>	11.7
Completeness [%]	92.6
Redundancy	6.3
CC(1/2)	0.996	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	100 mM sodium acetate, 1 M di-ammonium hydrogen phosphate (pH 4.5).