8K6P

Crystal structure of SHARPIN LTM motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-04-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	99.380, 39.240, 31.350
Unit cell angles	90.00, 97.04, 90.00

Refinement procedure

Resolution	25.200 - 1.860
R-factor	0.2208
Rwork	0.218
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.460	1.920
High resolution limit [Å]	1.860	1.860
Number of reflections	9723	866
<I/σ(I)>	10
Completeness [%]	98.2	87
Redundancy	5.9
CC(1/2)	0.994	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M sodium acetate trihydrate, and 2.0 M ammonium sulfate (pH 4.5).