8K6I

LnaB-Actin-PRUb ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 2 21 21
Unit cell lengths	57.377, 165.308, 224.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.330 - 3.190
R-factor	0.2933
Rwork	0.292
R-free	0.31480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.443
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.330	3.300
High resolution limit [Å]	3.190	3.190
Number of reflections	36304	1662
<I/σ(I)>	5.56
Completeness [%]	91.0
Redundancy	2
CC(1/2)	0.750	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	Sodium Chloride, PEG8000, HEPES