8K6F

LnaB-Actin-PRUb ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	54.690, 214.227, 79.563
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	37.290 - 3.410
R-factor	0.2236
Rwork	0.222
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.881
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.290	3.530
High resolution limit [Å]	3.410	3.410
Rmerge	0.130
Number of reflections	24455	1967
<I/σ(I)>	3.54
Completeness [%]	97.0
Redundancy	1.9
CC(1/2)	0.961	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	PEG8000, Sodium Choride, Sodium HEPES