8K6E

LnaB-Actin-PRUb ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979191
Spacegroup name	P 21 21 2
Unit cell lengths	215.317, 55.152, 80.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.680 - 2.740
R-factor	0.1912
Rwork	0.188
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.214
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.700	2.830
High resolution limit [Å]	2.740	2.740
Rmeas	0.069
Number of reflections	26157	25220
<I/σ(I)>	10.97
Completeness [%]	96.0
Redundancy	2
CC(1/2)	0.993	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	PEG 8000; Sodium chloride; Sodium HEPES