8K4Y
Structure of a triple-helix region of human ReCol 3 from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 17.867, 21.225, 89.138 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 44.606 - 1.500 |
| Rwork | 0.191 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.809 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.130 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 10965 | 539 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.994 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Li2SO4, 0.1M Bis-Tris pH6.5, 25% PEG3,350 |
