8K4X
Structure of a triple-helix region of human Collagen type XVII from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.936, 24.334, 60.814 |
| Unit cell angles | 90.00, 104.85, 90.00 |
Refinement procedure
| Resolution | 35.700 - 1.450 |
| Rwork | 0.173 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.133 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 18895 | 908 |
| <I/σ(I)> | 14.9 | 4.9 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.998 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium chloride, 0.05 M HEPES pH 7.5, 35% PEG 400 |
