8K4M

Anti CRISPR protein, AcrIIA13b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-03-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	102.510, 37.470, 37.090
Unit cell angles	90.00, 103.29, 90.00

Refinement procedure

Resolution	26.490 - 1.530
R-factor	0.2154
Rwork	0.214
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.490	1.585
High resolution limit [Å]	1.530	1.530
Number of reflections	19723	1949
<I/σ(I)>	18.82
Completeness [%]	94.2
Redundancy	7.2
CC(1/2)	1.000	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.2M Sodium Citrate pH6.1