8K4D

Structure of the SA2/Scc1/CENP_U complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	79.321, 132.615, 148.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 3.520
R-factor	0.22275
Rwork	0.220
R-free	0.26943
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.528
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.430	3.650
High resolution limit [Å]	3.520	3.520
Rmeas	0.059	0.740
Rpim	0.042	0.520
Number of reflections	19789	1830
<I/σ(I)>	10.39	1.59
Completeness [%]	99.2	93.27
Redundancy	2	2
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.03 M CaCl2,0.03 M MgCl2, 0.1 M MOPS-HEPES, pH7.5, 10% PEG8000, 20% ethylene glycol