8K4D
Structure of the SA2/Scc1/CENP_U complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.321, 132.615, 148.001 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.430 - 3.520 |
R-factor | 0.22275 |
Rwork | 0.220 |
R-free | 0.26943 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.528 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.430 | 3.650 |
High resolution limit [Å] | 3.520 | 3.520 |
Rmeas | 0.059 | 0.740 |
Rpim | 0.042 | 0.520 |
Number of reflections | 19789 | 1830 |
<I/σ(I)> | 10.39 | 1.59 |
Completeness [%] | 99.2 | 93.27 |
Redundancy | 2 | 2 |
CC(1/2) | 0.999 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.03 M CaCl2,0.03 M MgCl2, 0.1 M MOPS-HEPES, pH7.5, 10% PEG8000, 20% ethylene glycol |