8K3K

The crystal structure of nanobody Nb4 in complex with receptor binding domain (RBD) of BA.1 Spike protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	77.15
Detector technology	PIXEL
Collection date	2022-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	76.110, 76.110, 251.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.370 - 2.430
R-factor	0.2286
Rwork	0.224
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	AUTOMAR
Data scaling software	xia2
Phasing software	PHENIX (1.19.2-4158)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.370	2.490
High resolution limit [Å]	2.430	2.430
Number of reflections	30440	828
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	36.8
CC(1/2)	0.997	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M potassium thiocyanate, 20 %(w/v) PEG 3350