8K33
SOD1 and Nanobody1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-06 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.208, 119.670, 130.707 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 112.160 - 2.130 |
| R-factor | 0.2191 |
| Rwork | 0.217 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.160 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.131 | 0.693 |
| Number of reflections | 180626 | 27136 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 93.3 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291 | 0.2 M Ammonium acetate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350 |
