8K30

Crystal structure of a medaka mascRNA U23G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	157.940, 29.147, 99.179
Unit cell angles	90.00, 118.00, 90.00

Refinement procedure

Resolution	34.860 - 2.430
R-factor	0.2648
Rwork	0.264
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.071
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.020	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.054	0.813
Number of reflections	15588	1097
<I/σ(I)>	11.5	1.5
Completeness [%]	99.9	100
Redundancy	5.9
CC(1/2)	0.997	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	291	0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 18% v/v (+ / -)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride