8K2E

Crystal structure of YTHDC1 and Y3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	39.644, 103.515, 41.815
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	31.710 - 1.600
R-factor	0.1875
Rwork	0.185
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.331
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.70)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.094	0.081	0.449
Rmeas	0.102	0.088	0.494
Rpim	0.039	0.034	0.200
Total number of observations	291216
Number of reflections	43363	2220	2127
<I/σ(I)>	10.5
Completeness [%]	100.0	99.8	100
Redundancy	6.7	6.7	5.9
CC(1/2)	0.989	0.992	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris at pH 6.5, 0.2M ammonium sulfate, 25% PEG 3350