8K1E
Crystal structure of a human menRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.589, 29.193, 99.144 |
| Unit cell angles | 90.00, 126.75, 90.00 |
Refinement procedure
| Resolution | 36.250 - 2.230 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.591 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.440 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Number of reflections | 15793 | 1034 |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 99.2 | 90.3 |
| Redundancy | 5.5 | 3.4 |
| CC(1/2) | 0.904 | 0.371 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.04 M Lithium chloride, 0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 28% v/v (+ / -)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride |
