8JZY

RPA70N-RAD9 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97854
Spacegroup name	P 32 2 1
Unit cell lengths	50.041, 50.041, 93.255
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.260 - 1.500
R-factor	0.1856
Rwork	0.185
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv4
RMSD bond length	0.007
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20rc1_4392: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.260	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.553
Number of reflections	41852	2679
<I/σ(I)>	20.11
Completeness [%]	100.0	99.96
Redundancy	9.6
CC(1/2)	0.998	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 3350, sodium phosphate