8JZT
The sigF and anti-sigma factor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.531, 79.550, 77.598 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 38.780 - 1.940 |
| R-factor | 0.1705 |
| Rwork | 0.169 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.550 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.072 | 0.497 |
| Number of reflections | 60193 | 8747 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 6.5 and 23% w/v Polyethylene glycol 3,350 |
