8JZJ

E.coli Glyceraldehyde-3-phosphate dehydrogenase structure under cryoprotect condition of ammonium sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	277
Detector technology	CCD
Collection date	2022-06-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979340
Spacegroup name	I 41 2 2
Unit cell lengths	121.971, 121.971, 155.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.330 - 1.990
R-factor	0.1769
Rwork	0.175
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	2.050
High resolution limit [Å]	1.990	1.990
Rmerge	0.051	0.270
Rmeas	0.053	0.283
Rpim	0.016	0.085
Number of reflections	39834	2880
<I/σ(I)>	30.1	8.4
Completeness [%]	99.3	98.5
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2.7 M ammonium sulfate, 0.1 M MES