8JZH
C. glutamicum S-adenosylmethionine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 129.152, 203.886, 144.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.480 - 2.200 |
| R-factor | 0.18577 |
| Rwork | 0.183 |
| R-free | 0.22938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tde |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.077 | 0.060 | 0.377 |
| Rmeas | 0.084 | 0.067 | 0.413 |
| Rpim | 0.034 | 0.029 | 0.166 |
| Total number of observations | 560366 | ||
| Number of reflections | 95546 | 4887 | 4736 |
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 99.0 | 95.8 | 98.8 |
| Redundancy | 5.9 | 5.5 | 5.8 |
| CC(1/2) | 0.992 | 0.990 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Polyethylene glycol 200, MES NaOH pH 6.0, Polyethylene glycol 3000 |
