8JZD

Crystal structure of Escherichia coli NarJ in complex with the signal peptide of E. coli NarG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	H 3
Unit cell lengths	185.443, 185.443, 37.943
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.880 - 2.450
R-factor	0.2103
Rwork	0.208
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house model
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.068	0.617
Number of reflections	17555	871
<I/σ(I)>	31.3
Completeness [%]	98.1
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 8000, Tris