8JZA
Crystal structure of single domain antibody kt75 of human Thyroglobulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-03-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97777 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.751, 47.589, 64.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.239 - 1.350 |
Rwork | 0.130 |
R-free | 0.17440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.621 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.750 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.055 | 0.916 |
Rmeas | 0.059 | 0.981 |
Rpim | 0.021 | 0.349 |
Number of reflections | 26851 | 1329 |
<I/σ(I)> | 18 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.9 | 7.8 |
CC(1/2) | 0.999 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4M K-acetate, 0.1 M sodium HEPES pH 7.0, 10% glycerol |