8JYV

Crystal structure of the gasdermin from Trichoplax adhaerens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97927
Spacegroup name	P 31 2 1
Unit cell lengths	55.718, 55.718, 140.085
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.560 - 1.860
R-factor	0.2161
Rwork	0.214
R-free	0.23430
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250	1.910
High resolution limit [Å]	1.860	1.860
Rmerge	0.060	0.555
Rmeas	0.062	0.581
Rpim	0.014	0.164
Number of reflections	21681	1237
<I/σ(I)>	34.7	4.2
Completeness [%]	99.4	92.2
Redundancy	18.1	11.6
CC(1/2)	0.999	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	100 mM sodium citrate tribasic dihydrate pH 5.0, 18% polyethylene glycol 3550, 80 mM lithium nitrate