8JYV
Crystal structure of the gasdermin from Trichoplax adhaerens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2019-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.718, 55.718, 140.085 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.560 - 1.860 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.23430 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.555 |
| Rmeas | 0.062 | 0.581 |
| Rpim | 0.014 | 0.164 |
| Number of reflections | 21681 | 1237 |
| <I/σ(I)> | 34.7 | 4.2 |
| Completeness [%] | 99.4 | 92.2 |
| Redundancy | 18.1 | 11.6 |
| CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 100 mM sodium citrate tribasic dihydrate pH 5.0, 18% polyethylene glycol 3550, 80 mM lithium nitrate |
