8JYH

Crystal structure of engineered HIV-1 Reverse Transcriptase RNase H domain complexed with laccaic acid C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	61.850, 61.850, 82.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.730 - 2.210
R-factor	0.2335
Rwork	0.228
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	2.290
High resolution limit [Å]	2.210	2.210
Rmerge	0.115	1.293
Rmeas	0.120	1.346
Rpim	0.035	0.372
Total number of observations	106977	9360
Number of reflections	8440	730
<I/σ(I)>	9.3	1.4
Completeness [%]	99.7
Redundancy	12.7	12.8
CC(1/2)	0.995	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	291	0.1M MES, 26%(v/v) PEG6000, 0.01 M Zinc Sulfate, 0.001 M MANGANESE CHLORIDE