8JXZ

Chitin binding SusD-like protein AqSusD in complex with (GlcNAc)3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-29
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54184
Spacegroup name	C 1 2 1
Unit cell lengths	89.417, 64.465, 105.274
Unit cell angles	90.00, 113.38, 90.00

Refinement procedure

Resolution	19.330 - 2.200
R-factor	0.1901
Rwork	0.186
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.330	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	25384	2159
<I/σ(I)>	10.12
Completeness [%]	90.4
Redundancy	2.4
CC(1/2)	0.898	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium acetate, 0.1 M sodium cacodylate pH6.5, 20% (w/v) PEG 8000