8JX6

Deep-Sea Helicase 9 (DSH9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	56.808, 126.836, 150.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.100
R-factor	0.2027
Rwork	0.201
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.731
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.170
High resolution limit [Å]	2.060	2.060
Number of reflections	63166	8497
<I/σ(I)>	23
Completeness [%]	92.5
Redundancy	10.7
CC(1/2)	0.990	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Potassium Sodium tartrate tetrahydrate; 20% PEG 3350