8JVY

Crystal structure of the chimeric protein tkoL2_v1_turn with shorten loop 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	58.825, 58.825, 54.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.205 - 1.895
R-factor	0.2276
Rwork	0.226
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.895	1.895
Number of reflections	8373	1344
<I/σ(I)>	32.58
Completeness [%]	99.7
Redundancy	10.3
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 200mM Ammonium citrate tribasic pH7.5