8JVU

Crystal structure of the chimeric protein tkoL2_v1.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	32.718, 32.718, 76.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.335 - 2.505
R-factor	0.247
Rwork	0.245
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.106
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.500	2.500
Number of reflections	3109	477
<I/σ(I)>	12.74
Completeness [%]	100.0
Redundancy	5.3
CC(1/2)	0.990	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% 2-propanol, 100mM Tris-HCl pH8.5, 30% PEG 3350