8JVS

Crystal structure of the chimeric protein tkoL2_v1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	40.509, 40.509, 68.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.881 - 1.500
R-factor	0.201
Rwork	0.200
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	17462	2810
<I/σ(I)>	13.17
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2% PEG400, 100mM Tris pH8.5, 2000mM Lithium sulfate