8JV1
Structure of the SAR11 PotD in complex with GABA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.005, 78.348, 79.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.940 - 1.309 |
R-factor | 0.1233 |
Rwork | 0.122 |
R-free | 0.13800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | XDS (Jan 10, 2022 (BUILT 20220820)) |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.881 | 1.332 |
High resolution limit [Å] | 1.309 | 1.309 |
Rmeas | 0.049 | 0.698 |
Rpim | 0.014 | 0.248 |
Number of reflections | 105074 | 4870 |
<I/σ(I)> | 25.8 | 2.3 |
Completeness [%] | 99.2 | 92.8 |
Redundancy | 12.3 | 7.4 |
CC(1/2) | 0.999 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | The protein in complex with GABA was crystallized in drops containing 1 ul protein solution (60 mg/ml protein in buffer containing 10 mM HEPES, 150 mM NaCl, 50 mM GABA, pH 7.5) and 1 ul reservoir solution (100 mM MES pH 6.5, 20% w/v mPEG 550). |