8JUV

Crystal structure of a receptor like kinase with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	72.315, 72.315, 168.492
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.310 - 2.090
R-factor	0.1832
Rwork	0.181
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.630	2.210
High resolution limit [Å]	2.090	2.090
Number of reflections	28589	3042
<I/σ(I)>	13
Completeness [%]	92.5
Redundancy	5.1
CC(1/2)	0.994	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M lithium sulfate monohydrate, 0.1 M BIS-TRIS (pH 6.5), 25% (w/v) polyethylene glycol 3,350