8JUP

Crystal structure of a receptor like kinase from rice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	71.140, 71.140, 167.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.280 - 1.980
R-factor	0.2019
Rwork	0.201
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.171
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.870	2.030
High resolution limit [Å]	1.980	1.980
Number of reflections	35135	2556
<I/σ(I)>	9.8
Completeness [%]	99.9
Redundancy	17.7
CC(1/2)	0.996	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M lithium sulfate monohydrate, 0.1 M BIS-TRIS (pH 6.5), 25% (w/v) polyethylene glycol 3,350