8JUO

Crystal structure of aspartate semialdehyde dehydrogenase from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-03-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9796
Spacegroup name	I 21 21 21
Unit cell lengths	74.860, 108.860, 162.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 1.730
R-factor	0.2176
Rwork	0.216
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.750	1.790
High resolution limit [Å]	1.730	1.730
Rmerge		1.269
Number of reflections	69004	6845
<I/σ(I)>	19.59	2.03
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	296	0.2 M calcium acetate hydrate , 0.1 M MES: NaOH (pH 6) , 10 % (w/v) Isopropanol