8JTO
Outer membrane porin of Burkholderia pseudomallei (BpsOmp38) in complex with ceftazidime
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-04-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 6 |
Unit cell lengths | 151.045, 151.045, 49.099 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.250 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.749 |
Data reduction software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 2.250 |
High resolution limit [Å] | 2.249 | 2.250 |
Number of reflections | 27951 | 30807 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 87.4 | |
Redundancy | 2.3 | |
CC(1/2) | 0.993 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.5 M Sodium Chloride, 0.05 M Calcium Chloride dihydrate, 0.1 M MES, pH6.5, and 37% w/v PEG 400 |