8JTO

Outer membrane porin of Burkholderia pseudomallei (BpsOmp38) in complex with ceftazidime

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	110
Detector technology	CCD
Collection date	2020-04-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 6
Unit cell lengths	151.045, 151.045, 49.099
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 2.250
R-factor	0.1883
Rwork	0.187
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.749
Data reduction software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.250
High resolution limit [Å]	2.249	2.250
Number of reflections	27951	30807
<I/σ(I)>	11.1
Completeness [%]	87.4
Redundancy	2.3
CC(1/2)	0.993	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.5 M Sodium Chloride, 0.05 M Calcium Chloride dihydrate, 0.1 M MES, pH6.5, and 37% w/v PEG 400