8JTN

Tudor domain of TDRD3 in complex with a small molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-28
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	I 1 2 1
Unit cell lengths	27.862, 25.961, 80.355
Unit cell angles	90.00, 96.00, 90.00

Refinement procedure

Resolution	39.960 - 1.800
Rwork	0.206
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.960	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	5267	275
<I/σ(I)>	7.9
Completeness [%]	96.9
Redundancy	1.8
CC(1/2)	0.993	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1.2 M sodium citrate, 0.1 M Tris-HCl, pH 8.5