8JT3
Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in complex with amino donor L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.849, 113.280, 157.681 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 40.190 - 2.350 |
| R-factor | 0.19891 |
| Rwork | 0.197 |
| R-free | 0.23741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.667 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.129 | 0.593 |
| Rmeas | 0.150 | 0.685 |
| Rpim | 0.075 | 0.335 |
| Total number of observations | 322678 | 17008 |
| Number of reflections | 84776 | 4290 |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 93.7 | |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.989 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Sodium acetate, 0.1 M TRIS pH 8.5, 32%PEG 3350, 2% glycerol |
