8JST

GH11 family xylanase rMxylcd from the compost-soil metagenome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	67.010, 67.010, 139.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.290 - 1.500
R-factor	0.1629
Rwork	0.163
R-free	0.16460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.551
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.290	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.061	0.061
Number of reflections	131697	4770
<I/σ(I)>	20.8
Completeness [%]	93.6
Redundancy	18.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25% PEG 1500