8JSF

Crystal structure of a cytidylate cyclase from multidrug-resistant bacterium Elizabethkingia anopheles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97624
Spacegroup name	I 2 3
Unit cell lengths	164.483, 164.483, 164.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.380 - 2.200
R-factor	0.1809
Rwork	0.179
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2-4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	37544	3697
<I/σ(I)>	51	2.8
Completeness [%]	99.9	100
Redundancy	33.9
CC(1/2)	0.998	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.05 M Bis-Tris 7.0, 1.6 M Ammonium sulfate, 25% v/v Glycerol, 0.15 M Potassium chloride