8JS7
Dimeric PAS domains of oxygen sensor FixL in complex with imidazole-bound heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 188.854, 199.313, 270.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.830 - 2.850 |
R-factor | 0.2174 |
Rwork | 0.216 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.153 |
Data reduction software | XDS |
Data scaling software | XDS (20180808) |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.020 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmeas | 0.160 | 1.690 |
Number of reflections | 117838 | 36309 |
<I/σ(I)> | 10.5 | 1.04 |
Completeness [%] | 99.7 | 98 |
Redundancy | 7.21 | 7.25 |
CC(1/2) | 0.998 | 0.493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.5 M Ammonium sulfate, 0.1 M Sodium Acetate, 10 mM Imidazole |