8JRK

Crystal structure of the bat MHC II molecule at 2.3 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	91.946, 91.946, 214.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.110 - 2.300
R-factor	0.20918
Rwork	0.207
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.541
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.140	2.140
Rmerge	0.171	1.173
Number of reflections	49438	1612
<I/σ(I)>	14.4
Completeness [%]	99.2
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Potassium fluoride,20% Polyethylene glycol 3350,pH 7.3