8JQY

Crystal Structure of nucleotide-free mIRGB10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-03
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97957
Spacegroup name	P 32 2 1
Unit cell lengths	190.240, 190.240, 38.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.610 - 3.680
R-factor	0.2506
Rwork	0.249
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	3.812
High resolution limit [Å]	3.680	3.681
Number of reflections	8893	891
<I/σ(I)>	18.54
Completeness [%]	99.7	100
Redundancy	20.7
CC(1/2)	0.999	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.0 M ammonium sulfate, 0.2 M lithium sulfate, and 0.1 M Tris-HCl (pH 7.0)