8JQV

Hen egg-white lysozyme solved from 1D fixed target delivery system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	293
Detector technology	CCD
Collection date	2021-04-04
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.300, 79.300, 38.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.650 - 1.830
R-factor	0.2056
Rwork	0.204
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.879
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.830	1.830
Number of reflections	12462	1150
<I/σ(I)>	3.73	1.59
Completeness [%]	100.0	100
Redundancy	340.7	237.1
CC(1/2)	0.910	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4	291	0.1M sodium acetate pH4.0, 2M NaCl, 5% PEG 6K