8JPW

Crystal Structure of Single-chain L-Glutamate Oxidase Mutant from Streptomyces sp. X-119-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	158.400, 158.400, 138.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.320 - 2.660
Rwork	0.259
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	2.790
High resolution limit [Å]	2.660	2.660
Number of reflections	25525	3346
<I/σ(I)>	20
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.999	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293.15	0.1 M sodium acetate trihydrate (pH 4.5), 10% PEG3000 and 0.05 M zinc acetate dihydrate