8JPV

Glutamyl-tRNA synthetase from Methylacidiphilum fumariolicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2017-07-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5419
Spacegroup name	P 21 21 21
Unit cell lengths	40.310, 62.540, 190.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.140 - 2.510
R-factor	0.21174
Rwork	0.209
R-free	0.26574
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.316
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.140	7.940
High resolution limit [Å]	2.510	2.510
Rmerge		0.161
Number of reflections	16313	17211
<I/σ(I)>	9.5
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	HEPES buffer pH 7.0, 10% PEG 6000, 200 mM NaSCN