8JOW

Crystal structure of rabbit antibody with phosphorylated peptide bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-01
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	43.520, 43.560, 63.520
Unit cell angles	71.31, 84.39, 87.57

Refinement procedure

Resolution	29.860 - 1.400
R-factor	0.16321
Rwork	0.162
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.609
Data reduction software	MOSFLM (7.1.0)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.300	1.470
High resolution limit [Å]	1.400	1.400
Rmerge	0.092	0.368
Number of reflections	77744	10677
<I/σ(I)>	5.7	2.1
Completeness [%]	88.8
Redundancy	1.9
CC(1/2)	0.977	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.2 M Lithium chloride, 20 % w/v Polyethylene glycol 3350