8JOW
Crystal structure of rabbit antibody with phosphorylated peptide bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 43.520, 43.560, 63.520 |
Unit cell angles | 71.31, 84.39, 87.57 |
Refinement procedure
Resolution | 29.860 - 1.400 |
R-factor | 0.16321 |
Rwork | 0.162 |
R-free | 0.19930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.609 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.300 | 1.470 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.092 | 0.368 |
Number of reflections | 77744 | 10677 |
<I/σ(I)> | 5.7 | 2.1 |
Completeness [%] | 88.8 | |
Redundancy | 1.9 | |
CC(1/2) | 0.977 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.2 M Lithium chloride, 20 % w/v Polyethylene glycol 3350 |